@article{Batista2024,

title = {Effect of chiral silver nanoparticles on prolyl-oligopeptidase binding and activity},

author = {Carin C. S. Batista and Victor H. Toledo and Marcos P. C. Ramos and Vitor Oliveira and Javier Acuña and Marcelo Y. Icimoto and Iseli Lourenço Nantes-Cardoso},

url = {https://www.frontiersin.org/journals/nanotechnology/articles/10.3389/fnano.2024.1392694/full},

doi = {10.3389/fnano.2024.1392694},

issn = {2673-3013},

year  = {2024},

date = {2024-07-29},

urldate = {2024-07-29},

journal = {Front. Nanotechnol.},

volume = {6},

publisher = {Frontiers Media SA},

abstract = {Introduction: Silver nanoparticles have a diversity of applications both in biological and technological areas. More recently, studies conducted in the Nano/Bio interface have demonstrated that chiral nanocrystals grew in chiral templates, and nanostructures functionalized with chiral molecules present specific properties. These properties apply to advanced materials, energy, medicine, and pharmacology.



Methodology: The present study synthesized silver nanoparticles on silver seeds using D- and L-histidine as templates and borohydride as a reducing agent.



Results and Discussion: The nanoparticles were characterized by UV-visible spectroscopy and presented surface plasmon resonance (SPR) bands around 415 nm. CD spectra showed signals in the region of the SPR band, indicating the growth of nanocrystals with chiral distortion. Synthesized silver nanoparticles were also characterized by high-resolution transmission electron microscopy (HRTEM), which evidenced the presence of histidine corona. The silver nanoparticles were functionalized with prolyl-oligopeptidase (POP), a prolinespecific endopeptidase expressed in the brain. This enzyme cleaves neuroactive peptides involved in memory, learning, and neurodegeneration. The enzyme POP was expressed with a His-tag to provide competitive binding affinity to silver nanoparticles covered by D- and L-histidine. Considering the biological importance, POP was chosen as a model for studying the functionalization of chiral silver nanoparticles regarding the chiral discrimination for binding affinity and stabilization.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Martins-Nascimento2023,

title = {Virucidal action of photogenerated pink phenothiazine radical cation impregnated in cotton fabric and polypropylene face mask},

author = {Lilian S. Martins-Nascimento and Adrianne M.M. Brito and Julia D. Bronzato and Martha T. Oliveira and Silgia A. Costa and Sirlene M. Costa and Otaciro R. Nascimento and Iseli Lourenço Nantes-Cardoso},

url = {https://www.sciencedirect.com/science/article/abs/pii/S1010603023005750},

doi = {10.1016/j.jphotochem.2023.115110},

issn = {1010-6030},

year  = {2023},

date = {2023-11-00},

urldate = {2023-11-00},

journal = {Journal of Photochemistry and Photobiology A: Chemistry},

volume = {445},

publisher = {Elsevier BV},

abstract = {The COVID-19 pandemic increased the interest in the development of self-disinfecting materials. In this innovative study, cotton and polypropylene fabrics impregnated with the photochemically generated 10H-phenothiazine (PHT) radical cation (PHT•+) gained highly effective virucidal properties. The effectiveness of PHT•+ adsorption was demonstrated by advanced analytical techniques, including electron paramagnetic resonance (EPR), UV–visible, and FT-Raman spectroscopies that confirmed the presence of PHT•+ in the fabrics, even after washing and a re-exposure to light. Colorimetric test evidenced PHT•+ in the cotton fabric even after 2 months in the dark. Viral inactivation assays were performed with the fabrics treated with PHT. Results of qPCR showed that fabrics impregnated with PHT•+ were highly effective at reducing the viral genome compared to untreated fabrics. PHT•+ destroys the virus by an oxidative attack on the viral envelope, evidenced by the quenching of the EPR signal of fabrics exposed to a model of the virus envelope. Also, cryo-transmission electron microscopy captured stunning images of virus destruction caused by the oxidative attack of PHT•+ on the virus envelope. Overall, this study represents a significant advance in developing self-disinfecting materials and could have far-reaching implications for the fight against viral infections.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sobrinho2023,

title = {Oligomerization of photochemically oxidized phenothiazine by nanostructured <scp>\textit{α}‐Fe_{2}O_{3}</scp>},

author = {Luiza F Sobrinho and Otaciro R Nascimento and Marcelo Y Icimoto and Alexandre JC Lanfredi and Roosevelt Droppa and Iseli Lourenço Nantes-Cardoso},

url = {https://scijournals.onlinelibrary.wiley.com/doi/abs/10.1002/pi.6496},

doi = {10.1002/pi.6496},

issn = {1097-0126},

year  = {2023},

date = {2023-10-00},

urldate = {2023-10-00},

journal = {Polymer International},

volume = {72},

number = {10},

pages = {967--972},

publisher = {Wiley},

abstract = {Phenothiazines (PTZs) and their derivatives are a group of compounds that have diverse applications in medicine, the dye industry and advanced materials. Due to the electron–hole donating characteristic, these compounds have also been applied in batteries and solar energy devices. PTZs can be converted into stable radical cations by chemical and photochemical reactions. The PTZ radical cations are reactive for polymerization. The photochemical formation of PTZ radical cations is favored in aggregates by the Type I mechanism in which one molecule in the triplet excited state abstracts one electron from a neighbor molecule. In the present study, 10H-phenothiazine (PHT) was dissolved in chloroform and converted to the radical cation species (PHT•+) by UV light irradiation. The radical cation was characterized by UV–visible absorption and electron paramagnetic resonance techniques. Addition of α-Fe2O3 nanorods and nanowires to PHT•+ chloroform solution promoted its color change from orange to blue-green. Absorption measurement revealed a significant decrease of the UV and visible bands assigned to radical cation species and the appearance of a broad band at the spectral region of 650 nm. The blue-green solution was dried, leading to the formation of crystals that were characterized using the X-ray diffraction technique. Considering possible PHT oligo(poly)merization, the crystals were analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry that corroborated the formation of oligomers containing three, four and five PHT residues and their sulfoxide derivatives. The PHT oligomers retained photosensitivity and exhibited an electron paramagnetic resonance signal indicating possible applications as hole donors in electronic devices. Oligomerization was also obtained by aggregation of PHT•+ in sodium dodecylsulfate micelles indicating that proximity and orientation of the free radical molecules is the mechanism for the oligomerization. Therefore, a new route for PHT oligomerization catalyzed by α-Fe2O3 nanowires is proposed. The method is readily available, inexpensive and environmentally safe.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{deMiranda2023,

title = {Organic matrix-entrapped methylene blue as a photochemical reactor applied in chemical synthesis and nanotechnology},

author = {Érica G.A. de Miranda and Victor H. Toledo and Carolina G. dos Santos and Fanny Costa and Maria Diaz-Lopez and Thiago B. de Queiroz and Otaciro R. Nascimento and Iseli Lourenço Nantes-Cardoso},

url = {https://www.sciencedirect.com/science/article/abs/pii/S101060302300480X},

doi = {10.1016/j.jphotochem.2023.115015},

issn = {1010-6030},

year  = {2023},

date = {2023-10-00},

urldate = {2023-10-00},

journal = {Journal of Photochemistry and Photobiology A: Chemistry},

volume = {444},

publisher = {Elsevier BV},

abstract = {Aggregation modulates the photochemical and photophysical properties of methylene blue (MB+). MB+ adsorbed by the anionic polymer Chelex® 100 was characterized by UV–visible absorption spectroscopy and sulfur K-edge X-ray absorption near edge structure (XANES) as H- and J-aggregates. Nuclear magnetic resonance (NMR) indicates that dye molecules bind to the unprotonated carboxylic groups of the polymer via electrostatic interactions and are bridged by water molecules. MB+ also stacks with phenyl rings appended in the Chelex® 100 chain, leading to a gain in the mobility of ordered water displaced around the aromatic rings. Like the blue bottle experiment, MB+ in Chelex® 100 was entirely photoconverted for the leuco form (LMB) by visible and red light and reoxidized by molecular oxygen. Electron paramagnetic resonance (EPR) of MB+ in Chelex® 100 demonstrated the formation of a sulfur-centered free radical during photobleaching. The free radical involvement in MB+ photobleaching is consistent with a type I mechanism in which the stabilization of the products MB2+• and MB• in dye aggregates allows the formation of LMB by MB• dismutation or a second electron abstraction. Chelex® 100 beds loaded with MB+ solutions prepared in H2O and D2O produce H2O2 and D2O2, respectively, after successive cycles of photobleaching and recycling by O2 dissolved in the liquid phase. The formation of hydrogen peroxide is attributed to spontaneous superoxide ion disproportionation in the microenvironment of the polymer lattice. The LMB produced in Chelex® 100 was also able to act as a reducing agent for the synthesis of anisotropic iridescent gold nanoparticles (GNPs). The LMB/Chelex system allows the modulation of the shape and size of GNPs using different dye/Au3+ ratios. GNPs produced using LMB as a reducing agent are also efficient cell delivery systems. The specific properties of LMB confined in solid hosts LMB/Chelex also have potential applications in new solid-state optoelectronic devices such as sensing and imaging.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Guimarães2023,

title = {Rosemary (Rosmarinus officinalis L.) Glycolic Extract Protects Liver Mitochondria from Oxidative Damage and Prevents Acetaminophen-Induced Hepatotoxicity},

author = {Natalia S. S. Guimarães and Vyctória S. Ramos and Laura F. L. Prado-Souza and Rayssa M. Lopes and Gabriel S. Arini and Luís G. P. Feitosa and Ricardo R. Silva and Iseli Lourenço Nantes-Cardoso and Debora C. Damasceno and Norberto P. Lopes and Tiago Rodrigues},

url = {https://www.mdpi.com/2076-3921/12/3/628},

doi = {10.3390/antiox12030628},

issn = {2076-3921},

year  = {2023},

date = {2023-03-00},

urldate = {2023-03-00},

journal = {Antioxidants},

volume = {12},

number = {3},

publisher = {MDPI AG},

abstract = {Rosmarinus officinalis L. (rosemary) is an aromatic culinary herb. Native to the Mediterranean region, it is currently cultivated worldwide. In addition to its use as a condiment in food preparation and in teas, rosemary has been widely employed in folk medicine and cosmetics. Several beneficial effects have been described for rosemary, including antimicrobial and antioxidant activities. Here, we investigated the mechanisms accounting for the antioxidant activity of the glycolic extract of R. officinalis (Ro) in isolated rat liver mitochondria (RLM) under oxidative stress conditions. We also investigated its protective effect against acetaminophen-induced hepatotoxicity in vivo. A crude extract was obtained by fractionated percolation, using propylene glycol as a solvent due to its polarity and cosmeceutical compatibility. The quantification of substances with recognized antioxidant action revealed the presence of phenols and flavonoids. Dereplication studies carried out through LC-MS/MS and GC-MS, supported by The Global Natural Product Social Molecular Networking (GNPS) platform, annotated several phenolic compounds, confirming the previous observation. In accordance, Ro decreased the production of reactive oxygen species (ROS) elicited by Fe2+ or t-BOOH and inhibited the lipid peroxidation of mitochondrial membranes in a concentration-dependent manner in RLM. Such an effect was also observed in liposomes as membrane models. Ro also prevented the oxidation of mitochondrial protein thiol groups and reduced glutathione (GSH). In model systems, Ro exhibited a potent scavenger activity toward 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals and superoxide anions. It also demonstrated an Fe2+ chelating activity. Moreover, Ro did not exhibit cytotoxicity or dissipate the mitochondrial membrane potential (∆Ψ) in rat liver fibroblasts (BRL3A cells). To evaluate whether such antioxidant protective activity observed in vitro could also be achieved in vivo, a well-established model of hepatotoxicity induced by acute exposure to acetaminophen (AAP) was used. This model depletes GSH and promotes oxidative-stress-mediated tissue damage. The treatment of rats with 0.05% Ro, administered intraperitoneally for four days, resulted in inhibition of AAP-induced lipid peroxidation of the liver and the prevention of hepatotoxicity, maintaining alanine and aspartate aminotransferase (ALT/AST) levels equal to those of the normal, non-treated rats. Together, these findings highlight the potent antioxidant activity of rosemary, which is able to protect mitochondria from oxidative damage in vitro, and effects such as the antioxidant and hepatoprotective effects observed in vivo.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bronzato2023,

title = {Degradation of ciprofloxacin by green cobalt oxide quantum dots},

author = {Julia D. Bronzato and Juliana D. Bronzato and Adrianne M.M. Brito and Jefferson Bettini and Maicon R.Z. Passini and Brenda P.F.A. Gomes and Iseli Lourenço Nantes-Cardoso},

url = {https://www.sciencedirect.com/science/article/abs/pii/S0169433222027210},

doi = {10.1016/j.apsusc.2022.155193},

issn = {0169-4332},

year  = {2023},

date = {2023-01-00},

urldate = {2023-01-00},

journal = {Applied Surface Science},

volume = {609},

publisher = {Elsevier BV},

abstract = {Ciprofloxacin (CIPRO) is a fluoroquinolone antibiotic extensively used to treat various bacterial infections. The extensive use of CIPRO has promoted its accumulation in diverse aquatic environments as an emergent pollutant. Concentrations of CIPRO found in surface and ground waters and sea vary from ng to mg/L and represent a real risk for the rise of new resistant bacterial strains. The complex and stable chemical CIPRO structure is not favorable for biological degradation. However, magnetic Co3O4 quantum dots (Co3O4 QDs) with 5 nm of mean diameter synthesized using biomolecules from tomato (Solanum lycopersicum L.) seed extract were efficient in promoting photodegradation of this emergent pollutant under UV irradiation. UV–visible and Fourier Transform Infrared (FTIR) spectrophotometry corroborated CIPRO degradation. The photodegradation using UV light in the presence of Co3O4 QDs was the more efficient strategy to impair the bactericidal action of CIPRO and, consequently, avoid the development of bacteria-resistant strains. The Co3O4 QDs are a low-cost, efficient, and green option for CIPRO degradation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Toledo2022,

title = {Harvesting of Surfactant-Solubilized Asphaltenes by Magnetic Nanoparticles},

author = {Gabriel F. G. Toledo and James M. Almeida and Adrianne M. M. Brito and Carin C. S. Batista and Luana S. Andrade and Daniele R. de Araújo and Marcelo Y. Icimoto and S. Brochsztain and Iseli Lourenço Nantes-Cardoso},

url = {https://pubs.acs.org/doi/abs/10.1021/acs.energyfuels.2c01989},

doi = {10.1021/acs.energyfuels.2c01989},

issn = {1520-5029},

year  = {2022},

date = {2022-10-06},

urldate = {2022-10-06},

journal = {Energy Fuels},

volume = {36},

number = {19},

pages = {11839--11848},

publisher = {American Chemical Society (ACS)},

abstract = {Asphaltenes are a severe problem for the oil industry. The high content of aromatic and aliphatic hydrocarbons in asphaltenes poses a challenge for efficient methods of the solubilization and degradation of their components. The main goal of this study was to investigate an efficient and innovative method for asphaltene solubilization with surfactants to produce supramolecular aggregates with affinity by magnetic nanoparticles (Fe3O4) for magnetic separation and degradation. Asphaltene mixed with the cationic surfactant cetyltrimethylammonium bromide (CTAB) was both solubilized in chloroform and the solvent dried with N2 to produce a film that was resuspended in water and formed a stable colloid with asphaltene incorporated in CTAB micelles. The suspensions of CTAB/asphaltene supramolecular aggregates obtained at different surfactant/asphaltene ratios were characterized by dynamic and static light scattering (DLS and SLS) and by electrophoretic mobility for ζ potential determination. CTAB concentrations of 30 and 60 mM produced spherical supramolecular aggregates (SMAs) of size between 100 and 200 nm with polydispersity. The ζ potential of CTAB micelles loaded with asphaltenes increased from +9.17 +/– 4.6 to +56.7 +/– 5.8 eV. Electron paramagnetic resonance revealed that asphaltene forms stable free radicals in CTAB micelles. Classical molecular dynamics simulations were also used to study interactions of the functional groups of asphaltenes. The association with CTAB micelles provided the binding affinity of asphaltenes for nanoparticulate magnetite (Fe3O4) and precipitation of the most CTAB content. In this condition, Fe3O4 promoted the degradation of asphaltenes to low molecular mass products. Therefore, incorporation in CTAB micelles is a simple and innovative method contributing to asphaltene removal, degradation, and possible conversion to products with aggregated value.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Araujo-Chaves2021,

title = {Antioxidant cytochrome c-like activity of para-Mn(III)TMPyP},

author = {Juliana C. Araujo-Chaves and Érica G.A. Miranda and David M. Lopes and César H. Yokomizo and Waldemir M. Carvalho-Jr and Iseli Lourenço Nantes-Cardoso},

url = {https://www.sciencedirect.com/science/article/pii/S0300908421000663},

doi = {10.1016/j.biochi.2021.02.015},

issn = {0300-9084},

year  = {2021},

date = {2021-05-00},

urldate = {2021-05-00},

journal = {Biochimie},

volume = {184},

pages = {116--124},

publisher = {Elsevier BV},

abstract = {Manganese porphyrins are well-known protectors against the deleterious effects of pro-oxidant species such as superoxide ions and hydrogen peroxide. The present study investigated the antioxidant cytochrome c-like activities of Mn(III)TMPyP [meso-tetrakis (4-N-methyl pyridinium) porphyrin] against superoxide ion and hydrogen peroxide that remained unexplored for this porphyrin. The association of TMPyP with a model of the inner mitochondrial membrane, cardiolipin (CL)-containing liposomes, shifted +30 mV vs. NHE (normal hydrogen electrode) redox potential of the Mn(II)/Mn(III) redox couple. In CL-containing liposomes, Mn(III)TMPyP was reduced by superoxide ions and recycled by Fe(III)cytochrome c to the oxidized form. Similarly, isolated rat liver mitoplasts added to a sample of Mn(II)TMPyP promoted immediate porphyrin reoxidation by electron transfer to the respiratory chain. These results show that Mn(III)TMPyP can act as an additional pool of Fe(III)cytochrome c capable of transferring electrons that escape from the IV complex back into the respiratory chain. Unlike Fe(II)cytochrome c, Mn(II)TMPyP was not efficient for hydrogen peroxide clearance. Therefore, by reducing cytochrome c, Mn(II)TMPyP can indirectly contribute to hydrogen peroxide elimination.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Santos2021c,

title = {TECNOLOGIA E CULTURA NO ENSINO DE QUÍMICA: UM ENFOQUE MULTIDISCIPLINAR SOBRE O USO DE VÍDEOS EM SALA DE AULA / TECHNOLOGY AND CULTURE IN CHEMISTRY TEACHING: A MULTIDISCIPLINARY FOCUS ON THE USE OF VIDEO IN THE CLASSROOM},

author = {Hebert Freitas dos Santos and Iseli Lourenço Nantes-Cardoso},

url = {https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/24196},

doi = {10.34117/bjdv7n2-049},

issn = {2525-8761},

year  = {2021},

date = {2021-00-00},

urldate = {2021-00-00},

journal = {BJD},

volume = {7},

number = {2},

pages = {12454--12474},

publisher = {Brazilian Journal of Development},

abstract = {Este trabalho tem por objetivo mostrar e exemplificar as potencialidades do uso de vídeos como estratégia para construção do conhecimento e avaliação e algumas de suas relações com a BNCC (Base Nacional Comum Curricular), expor como professores e graduandos participantes do curso Tecnologia e Cultura no Ensino de Química, realizado na Universidade Federal do ABC (UFABC) em 2018, enxergavam a aplicação deste meio como forma de complementar seu plano de aula, bem como apresentar alguns programas de edição de vídeos e os direitos autorais pertinentes à educação para uso e produção de material audiovisual. Como resultado no que tange ao curso, os cursistas sentiram-se mais motivados em produzir material de própria autoria e perceberam o potencial pedagógico do vídeo como instrumento para aulas mais interativas e trabalhos diferenciados. Além disso, espera-se que o leitor, professor de qualquer segmento e disciplina, encontre o suporte necessário, incluindo um modelo de plano de aula, para que introduza esta ferramenta que ganhou tamanha importância em virtude da pandemia, mas que tem seu valor pedagógico em qualquer situação.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}